Regulatory

Part:BBa_J119371:Design

Designed by: MWSU Genetics Fall 2014 Tuesday 2:00 lab   Group: Eckdahl Lab   (2014-11-07)


Ptac mutant 15 A's inserted after -35 region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Left sticky end of CGAC and right sticky end of CCGC. No BsaI sites allowed.


Source

Synthetic oligonucleotides.

References